PDE8A Antibody / Phosphodiesterase 8A
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
O60658
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human PDE8A recombinant protein (Position: R32-E495) was used as the immunogen for the PDE8A antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This PDE8A antibody is available for research use only.
Storage Conditions
After reconstitution, the PDE8A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Product Datasheet
http://www.nsjbio.com/tds-pdf/pde8a-antibody-phosphodiesterase-8a-fy12544-nsj-bioreagents
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the PDE8A antibody should be determined by the researcher.
Image Legend
Flow Cytometry analysis of SH-SY5Y cells using anti-PDE8A antibody. Overlay histogram showing SH-SY5Y cells stained with (Blue line) . To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE8A antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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