ITGB7 Antibody / Integrin beta 7
Product Specifications
Specifications
Western blot: 1:500-1:2000
UniProt
P26010
Host
Rabbit
Reactivity
Human
Immunogen
A synthesized peptide derived from human Integrin beta 7 was used as the immunogen for the ITGB7 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
32I45
Applications
WB
Purity
Affinity-chromatography
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.
Limitations
This ITGB7 antibody is available for research use only.
Storage Conditions
Store the ITGB7 antibody at -20°C.
Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the ITGB7 antibody should be determined by the researcher.
Location
Cytoplasm, cell membrane
Image Legend
Immunohistochemical staining of ITGB7 using anti-ITGB7 antibody. ITGB7 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ITGB7 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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