Phospho-c-Jun/JunD (Ser73/Ser100) Antibody
Product Specifications
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:50
UniProt
P05412, P17535
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A synthesized peptide derived from human Phospho-c-Jun (S73) +JunD (S100) was used as the immunogen for the Phospho-c-Jun/JunD (Ser73/Ser100) antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
30J61
Applications
WB, IHC, IP
Purity
Affinity-chromatography
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.
Limitations
This Phospho-c-Jun/JunD (Ser73/Ser100) antibody is available for research use only.
Storage Conditions
Store the Phospho-c-Jun/JunD (Ser73/Ser100) antibody at -20°C.
Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the Phospho-c-Jun/JunD (Ser73/Ser100) antibody should be determined by the researcher.
Image Legend
Western blot analysis of Phospho-c-Jun (S73) +JunD (S100) expression in mouse RAW 264.7 cell lysate using Phospho-c-Jun/JunD (Ser73/Ser100) antibody. Phospho-c-Jun/JunD (Ser73/Ser100) was detected in RAW264.7 lysate as a ~38-45 kDa doublet, consistent with JNK/MAPK-dependent multi-site phosphorylation of c-Jun and JunD that produces reduced-mobility bands on SDS-PAGE.
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