RTRAF Antibody / hCLE

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

Q9Y224

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human C14orf166/RTRAF recombinant protein (Position: M1-R244) was used as the immunogen for the RTRAF antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ICC/IF, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This RTRAF antibody is available for research use only.

Storage Conditions

After reconstitution, the RTRAF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the RTRAF antibody should be determined by the researcher.

Location

Cytoplasmic, Nuclear

Image Legend

Immunohistochemical staining of RTRAF using anti-RTRAF antibody. RTRAF was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RTRAF antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.