TCIRG1 Antibody / T-cell immune regulator 1

After reconstitution, the TCIRG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunohistochemical staining of TCIRG1 using anti-TCIRG1 antibody. TCIRG1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TCIRG1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate. Tonsil section shows scattered cytoplasmic/vesicular staining in macrophages and histiocytes within the germinal centers, consistent with TCIRG1 expression in antigen-presenting phagocytes.