Phospho-CDK2/CDK1 (Thr160/Thr161) Antibody

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

UniProt

P24941, P06493

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human Phospho-CDK2 (T160) +CDK1 (T161) was used as the immunogen for the Phospho-CDK2/CDK1 (Thr160/Thr161) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31C95

Applications

WB, IHC

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Phospho-CDK2/CDK1 (Thr160/Thr161) antibody is available for research use only.

Storage Conditions

Store the Phospho-CDK2/CDK1 (Thr160/Thr161) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-CDK2/CDK1 (Thr160/Thr161) antibody should be determined by the researcher.

Location

Cytoplasm

Image Legend

Immunohistochemical staining of CDK2 (Phospho-T160) /CDK1 (Phospho-T161) using anti-Phospho-CDK2/CDK1 (Thr160/Thr161) antibody. CDK2 (Phospho-T160) /CDK1 (Phospho-T161) was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Phospho-CDK2/CDK1 (Thr160/Thr161) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.