AKT1/2/3 Antibody
Product Specifications
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50
UniProt
P31749, P31751, Q9Y243
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human AKT1 + AKT2 + AKT3 was used as the immunogen for the AKT1/2/3 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
31A24
Applications
WB, IHC, ICC, IF, IP, FACS
Purity
Affinity-chromatography
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.
Limitations
This AKT1/2/3 antibody is available for research use only.
Storage Conditions
Store the AKT1/2/3 antibody at -20°C.
Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the AKT1/2/3 antibody should be determined by the researcher.
Image Legend
All lanes use the AKT1/2/3 antibody at 1:5000 dilution for 1 hour at room temperature. Western blot analysis of AKT1/2/3 using a pan-AKT antibody. A principal band is detected at ~56 kDa with a faint higher-migrating band consistent with phosphorylated AKT and a very light lower species consistent with a less-phosphorylated or partially processed form.
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