AKT1/2/3 Antibody

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

UniProt

P31749, P31751, Q9Y243

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human AKT1 + AKT2 + AKT3 was used as the immunogen for the AKT1/2/3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31A24

Applications

WB, IHC, ICC, IF, IP, FACS

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This AKT1/2/3 antibody is available for research use only.

Storage Conditions

Store the AKT1/2/3 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the AKT1/2/3 antibody should be determined by the researcher.

Image Legend

All lanes use the AKT1/2/3 antibody at 1:5000 dilution for 1 hour at room temperature. Western blot analysis of AKT1/2/3 using a pan-AKT antibody. A principal band is detected at ~56 kDa with a faint higher-migrating band consistent with phosphorylated AKT and a very light lower species consistent with a less-phosphorylated or partially processed form.