HIF1A Antibody / HIF-1 alpha

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunocytochemistry/Immunofluorescence: 1:50-1:200

UniProt

Q16665

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human HIF-1-alpha was used as the immunogen for the HIF1A antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31H88

Applications

WB, ICC, IF

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This HIF1A antibody is available for research use only.

Storage Conditions

Store the HIF1A antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the HIF1A antibody should be determined by the researcher.

Image Legend

Western blot analysis of HIF-1-Alpha using anti-HIF1A antibody. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1A antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Predicted molecular weight ~110 kDa, commonly observed at 100-120 kDa.