S100P Antibody / Protein S100-P

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P25815

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human S100P was used as the immunogen for the S100P antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC, IF, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This S100P antibody is available for research use only.

Storage Conditions

After reconstitution, the S100P antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the S100P antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of S100P using anti-S100P antibody (green) and anti-Beta Tubulin antibody (red) . S100P was detected in immunocytochemical section of cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-S100P antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and DyLight 594 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.