POLR1E Antibody / DNA-directed RNA polymerase I subunit E / PAF53
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL
UniProt
Q9GZS1
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human POLR1E recombinant protein (Position: M1-Q291) was used as the immunogen for the POLR1E antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ICC/IF, IP, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This POLR1E antibody is available for research use only.
Storage Conditions
After reconstitution, the POLR1E antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the POLR1E antibody should be determined by the researcher.
Location
Nucleus, Nucleolus
Image Legend
Immunofluorescent staining of POLR1E using anti-POLR1E antibody (green) and anti-Beta Tubulin antibody (red) . POLR1E was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-POLR1E antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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