STX10 Antibody / Syntaxin 10
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
O60499
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human Syntaxin 10/STX10 recombinant protein (Position: K77-K218) was used as the immunogen for the STX10 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This STX10 antibody is available for research use only.
Storage Conditions
After reconstitution, the STX10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Product Datasheet
http://www.nsjbio.com/tds-pdf/stx10-antibody-syntaxin-10-fy12767-nsj-bioreagents
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the STX10 antibody should be determined by the researcher.
Location
Golgi apparatus
Image Legend
Immunofluorescent staining of Syntaxin 10/STX10 using anti-STX10 antibody (green) . Syntaxin 10/STX10 was detected in immunocytochemical section of cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/ml rabbit anti-STX10 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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