NDUFA1 Antibody / NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

O15239

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human NDUFA1 recombinant protein (Position: M1-D70) was used as the immunogen for the NDUFA1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This NDUFA1 antibody is available for research use only.

Storage Conditions

After reconstitution, the NDUFA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the NDUFA1 antibody should be determined by the researcher.

Location

Cytoplasm (Mitochondria)

Image Legend

Western blot analysis of NDUFA1 using anti-NDUFA1 antibody. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band is detected at ~10-11 kDa, consistent with the known slower SDS-PAGE migration of this small, hydrophobic Complex I subunit relative to its ~7-8 kDa calculated mass.