SPIDR Antibody / Scaffold protein involved in DNA repair

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q14159

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human SPIDR recombinant protein (Position: E273-E904) was used as the immunogen for the SPIDR antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IF, ICC, WB, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This SPIDR antibody is available for research use only.

Storage Conditions

After reconstitution, the SPIDR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the SPIDR antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Immunofluorescent staining of SPIDR using anti-SPIDR antibody (red) and anti-Beta Tubulin antibody (green) . SPIDR was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SPIDR antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and FITC Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.