APOBEC3C Antibody
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
Q9NRW3
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human APOBEC3C was used as the immunogen for the APOBEC3C antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, ICC, IF, FACS
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This APOBEC3C antibody is available for research use only.
Storage Conditions
After reconstitution, the APOBEC3C antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the APOBEC3C antibody should be determined by the researcher.
Location
Nuclear, cytoplasmic
Image Legend
Immunofluorescent staining of APOBEC3C using anti-APOBEC3C antibody (green) and anti-Beta Tubulin antibody (red) . APOBEC3C was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-APOBEC3C antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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