ITGA4 Antibody / Integrin Alpha 4

After reconstitution, the ITGA4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of Integrin Alpha 4/ITGA4 using anti-ITGA4 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A broad band is detected at ~150 kDa in most samples, consistent with the mature glycosylated alpha 4 subunit; U87 shows a prominent ~130-140 kDa band with a minor lower species, consistent with cell-type-dependent glycosylation and limited processing relative to the ~115 kDa core mass.