IRAK4 Antibody / Interleukin-1 receptor-associated kinase 4

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

Q9NWZ3

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human IRAK4 recombinant protein (Position: N15-S460) was used as the immunogen for the IRAK4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This IRAK4 antibody is available for research use only.

Storage Conditions

After reconstitution, the IRAK4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the IRAK4 antibody should be determined by the researcher.

Image Legend

Western blot analysis of IRAK4 using anti-IRAK4 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRAK4 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A major band is observed at ~52 kDa corresponding to full-length IRAK4. In THP-1 lysates, an additional lower-migrating band appears, consistent with a mixture of phosphorylated and non-phosphorylated forms of IRAK4 reported in activated myeloid cells.