NDUFAB1 Antibody / NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 1

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:50, Flow Cytometry: 1:50

UniProt

O14561

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human NDUFAB1 was used as the immunogen for the NDUFAB1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

30N94

Applications

WB, IHC, IP, FACS

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This NDUFAB1 antibody is available for research use only.

Storage Conditions

Store the NDUFAB1 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the NDUFAB1 antibody should be determined by the researcher.

Location

Cytoplasm (Mitochondria)

Image Legend

Immunohistochemical staining of NDUFAB1 using anti-NDUFAB1 antibody. NDUFAB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NDUFAB1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.