PIK3C2A Antibody / Phosphoinositide 3-kinase-C2-alpha
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL
UniProt
O00443
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
E.coli-derived human PIK3C2A recombinant protein (Position: R22-E1523) was used as the immunogen for the PIK3C2A antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ICC/IF, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This PIK3C2A antibody is available for research use only.
Storage Conditions
After reconstitution, the PIK3C2A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the PIK3C2A antibody should be determined by the researcher.
Location
Cytoplasm, Nucleus, Cell membrane
Image Legend
Immunofluorescent staining of PIK3C2A using anti-PIK3C2A antibody (green) . PIK3C2A was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-PIK3C2A antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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