TNS3 Antibody / Tensin 3

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q68CZ2

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human TNS3 recombinant protein (Position: Q592-E869) was used as the immunogen for the TNS3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC, ICC, IF, IP, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This TNS3 antibody is available for research use only.

Storage Conditions

After reconstitution, the TNS3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the TNS3 antibody should be determined by the researcher.

Location

Cytoplasm, cell junctions

Image Legend

Immunofluorescent staining of TNS3 using anti-TNS3 antibody. TNS3 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-TNS3 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . TNS3 localizes to focal adhesions as peripheral puncta and short streaks at stress-fiber termini, consistent with its focal-adhesion scaffold role.