GALNT16 Antibody / Polypeptide N-acetylgalactosaminyltransferase 16

After reconstitution, the GALNT16 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of GALNT16 using anti-GALNT16 antibody. Lane 1: human 293T whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT16 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. GALNT16 western blot across human cell lines and rodent brain shows the expected band near ~65-70 kDa and an additional ~90 kDa band that is prominent in brain. The upper species likely represents a more heavily glycosylated and/or oligomeric form of GALNT16, consistent with known processing and complex formation of ppGalNAc-T family glycosyltransferases.