CACNA1A Antibody / Calcium voltage-gated channel subunit alpha1 A / Cav2.1

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

O00555

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

E.coli-derived human CACNA1A recombinant protein (Position: K251-D2157) was used as the immunogen for the CACNA1A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This CACNA1A antibody is available for research use only.

Storage Conditions

After reconstitution, the CACNA1A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the CACNA1A antibody should be determined by the researcher.

Image Legend

Western blot analysis of CACNA1A using anti-CACNA1A antibody. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA1A antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. CACNA1A western blot of rat and mouse brain shows a broad band at ~300-320 kDa, consistent with full-length Cav2.1 running higher than predicted due to extensive glycosylation and large membrane protein migration properties.