NKR1 Antibody / Neurokinin 1 Receptor / TACR1

Product Specifications

Specifications

Immunoprecipitation: 2-4ug/500ug of lysate, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

P25103

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Neurokinin 1 Receptor was used as the immunogen for the NKR1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

AFBH-20

Applications

IP, IHC, WB

Purity

Affinity-chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This NKR1 antibody is available for research use only.

Storage Conditions

Store the NKR1 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the NKR1 antibody should be determined by the researcher.

Location

Cytoplasm, cell membrane

Image Legend

Immunohistochemical staining of Neurokinin 1 Receptor using anti-NKR1 antibody. Neurokinin 1 Receptor was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-NKR1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.