NOLC1 Antibody / Nucleolar and coiled-body phosphoprotein 1 / NOPP140

Western blot analysis of NOLC1 using anti-NOLC1 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOLC1 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. NOLC1 western blot across human cell lines shows a predominant band at ~130 kDa. Although the predicted mass is ~74 kDa, NOLC1/Nopp140 is highly acidic and heavily phosphorylated, causing it to migrate markedly above its theoretical size on SDS-PAGE.