SHE Antibody / SH2 domain-containing adapter protein E
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q5VZ18
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
E.coli-derived human SHE recombinant protein (Position: L36-H495) was used as the immunogen for the SHE antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, IF, ICC/IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This SHE antibody is available for research use only.
Storage Conditions
After reconstitution, the SHE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the SHE antibody should be determined by the researcher.
Location
Cytoplasmic, Nuclear
Image Legend
Immunofluorescent staining of SHE using anti-SHE antibody (red) . SHE was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-SHE antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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