IL10 Antibody / Interleukin 10

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

P22301

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Interleukin-10 was used as the immunogen for the IL10 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

AOIG-9

Applications

WB, ICC, IF, FACS

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This IL10 antibody is available for research use only.

Storage Conditions

Store the IL10 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the IL10 antibody should be determined by the researcher.

Image Legend

Western blot analysis of IL10 using anti-IL10 antibody. Lane 1: human U251 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot of IL-10 in human and rodent spleen lysates shows a band at ~17-18 kDa, consistent with the mature, processed IL-10 monomer after removal of the signal peptide. The apparent size is slightly lower than the 21 kDa precursor predicted from the unprocessed sequence.