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PRAG1 Antibody / Pragmin / PEAK1-related kinase-activating pseudokinase 1

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q86YV5

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human PRAG1 recombinant protein (Position: H2-V1104) was used as the immunogen for the PRAG1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC, IF, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This PRAG1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PRAG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

http://www.nsjbio.com/tds-pdf/prag1-antibody-pragmin-peak1-related-kinase-activating-pseudokinase-1-fy12864-nsj-bioreagents

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the PRAG1 antibody should be determined by the researcher.

Location

Nucleus, Cell Junctions, possible Cytoplasm

Image Legend

Immunofluorescent staining of PRAG1 using anti-PRAG1 antibody (red) . PRAG1 was detected in an immunocytochemical section of human HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-PRAG1 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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