PRAG1 Antibody / Pragmin / PEAK1-related kinase-activating pseudokinase 1
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q86YV5
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human PRAG1 recombinant protein (Position: H2-V1104) was used as the immunogen for the PRAG1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, ICC, IF, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This PRAG1 antibody is available for research use only.
Storage Conditions
After reconstitution, the PRAG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the PRAG1 antibody should be determined by the researcher.
Location
Nucleus, Cell Junctions, possible Cytoplasm
Image Legend
Immunofluorescent staining of PRAG1 using anti-PRAG1 antibody (red) . PRAG1 was detected in an immunocytochemical section of human HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-PRAG1 antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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