SLC27A2 Antibody / FATP2

After reconstitution, the SLC27A2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of SLC27A2 using anti-SLC27A2 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse liver tissue lysates Lane 7: mouse kidney tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC27A2 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. SLC27A2 western blot across tissues shows the expected ~70 kDa band and a consistent lower band in mouse and rat kidney, consistent with co-expression of the FATP2a and FATP2b splice isoforms and minor PTM-dependent mobility differences.