CXCL13 Antibody / C-X-C motif chemokine 13 / BCA1
Selected Variant
FY12908
Size: 100 µg
Product Specifications
Specifications
Flow cytometry: 1-3ug/million cells, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
O43927
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CXCL13 was used as the immunogen for the CXCL13 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
FACS, IHC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This CXCL13 antibody is available for research use only.
Storage Conditions
After reconstitution, the CXCL13 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the CXCL13 antibody should be determined by the researcher.
Location
Cytoplasmic, secreted
Image Legend
Immunohistochemical staining of CXCL13 using anti-CXCL13 antibody. CXCL13 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CXCL13 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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