CXCL13 Antibody / C-X-C motif chemokine 13 / BCA1

Product Specifications

Specifications

Flow cytometry: 1-3ug/million cells, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

O43927

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human CXCL13 was used as the immunogen for the CXCL13 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

FACS, IHC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This CXCL13 antibody is available for research use only.

Storage Conditions

After reconstitution, the CXCL13 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the CXCL13 antibody should be determined by the researcher.

Location

Cytoplasmic, secreted

Image Legend

Immunohistochemical staining of CXCL13 using anti-CXCL13 antibody. CXCL13 was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CXCL13 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.