IER3 Antibody / Immediate early response gene 3
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P46695
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human IER3 was used as the immunogen for the IER3 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC, FACS
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This IER3 antibody is available for research use only.
Storage Conditions
After reconstitution, the IER3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the IER3 antibody should be determined by the researcher.
Image Legend
Immunohistochemical staining of IER3 using anti-IER3 antibody. IER3 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-IER3 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
PKSM041047-01 10 µg
Recombinant Mouse IL1R1/CD121a Protein (Fc Tag)
Sign In for Pricing
View Details PKSM041047-02 50 µg
Recombinant Mouse IL1R1/CD121a Protein (Fc Tag)
Sign In for Pricing
View Details 
BNCB2956-100 1x 100 µL
C1QA / Complement C1q A-Chain (C1QA/2956), Biotin conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
