Phospho-CrkL (Tyr207) Antibody / Crk like protein
Product Specifications
Specifications
Western blot: 1:500-1:2000
UniProt
P46109
Host
Rabbit
Reactivity
Human
Immunogen
A synthesized peptide derived from human Phospho-CrkL (Y207) was used as the immunogen for the Phospho-CrkL (Tyr207) antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
32C08
Applications
WB
Purity
Affinity chromatography
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.
Limitations
This Phospho-CrkL (Tyr207) antibody is available for research use only.
Storage Conditions
Store the Phospho-CrkL (Tyr207) antibody at -20°C.
Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the Phospho-CrkL (Tyr207) antibody should be determined by the researcher.
Image Legend
Human K562 cell lysate tested with the Phospho-CrkL (Tyr207) antibody at 1:1000 dilution for 1 hour at room temperature. A tight doublet is detected at ~40 kDa in K562 cells, running above the ~34 kDa predicted mass. The upward shift and doublet pattern are consistent with mono- and hyper-phosphorylated CrkL species, which migrate slower on SDS-PAGE than total CrkL.
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