Phospho-CrkL (Tyr207) Antibody / Crk like protein

Product Specifications

Specifications

Western blot: 1:500-1:2000

UniProt

P46109

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-CrkL (Y207) was used as the immunogen for the Phospho-CrkL (Tyr207) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32C08

Applications

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Phospho-CrkL (Tyr207) antibody is available for research use only.

Storage Conditions

Store the Phospho-CrkL (Tyr207) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-CrkL (Tyr207) antibody should be determined by the researcher.

Image Legend

Human K562 cell lysate tested with the Phospho-CrkL (Tyr207) antibody at 1:1000 dilution for 1 hour at room temperature. A tight doublet is detected at ~40 kDa in K562 cells, running above the ~34 kDa predicted mass. The upward shift and doublet pattern are consistent with mono- and hyper-phosphorylated CrkL species, which migrate slower on SDS-PAGE than total CrkL.