TAOK1 Antibody / TAO kinase 1

After reconstitution, the TAOK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Immunoprecipitating TAOK1 in Hela whole cell lysate. Western blot analysis of TAOK1 using anti-TAOK1 antibody. Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TAOK1 antibody in Hela whole cell lysate, Lane 3: anti-TAOK1 antibody (2ug) + Hela whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TAOK1 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A predominant band is detected at ~130 kDa, higher than the ~116 kDa prediction. The upward shift is consistent with hyperphosphorylated TAOK1, which migrates slower on SDS-PAGE.