TAOK1 Antibody / TAO kinase 1

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q7L7X3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human TAOK1 recombinant protein (Position: Q424-R884) was used as the immunogen for the TAOK1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IP, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This TAOK1 antibody is available for research use only.

Storage Conditions

After reconstitution, the TAOK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the TAOK1 antibody should be determined by the researcher.

Image Legend

Immunoprecipitating TAOK1 in Hela whole cell lysate. Western blot analysis of TAOK1 using anti-TAOK1 antibody. Lane 1: Hela whole cell lysates (30ug), Lane 2: Rabbit control IgG instead of anti-TAOK1 antibody in Hela whole cell lysate, Lane 3: anti-TAOK1 antibody (2ug) + Hela whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TAOK1 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A predominant band is detected at ~130 kDa, higher than the ~116 kDa prediction. The upward shift is consistent with hyperphosphorylated TAOK1, which migrates slower on SDS-PAGE.