GALNT2 Antibody / Polypeptide N-acetylgalactosaminyltransferase 2

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, ELISA: 0.1-0.5 µg/mL

UniProt

Q10471

Host

Rabbit

Reactivity

Human, Rat

Immunogen

E.coli-derived human GALNT2 recombinant protein (Position: H442-Q571) was used as the immunogen for the GALNT2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IP, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This GALNT2 antibody is available for research use only.

Storage Conditions

After reconstitution, the GALNT2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the GALNT2 antibody should be determined by the researcher.

Image Legend

Immunoprecipitating (IP) GALNT2 in Hela whole cell lysate. Western blot analysis of GALNT2 using anti-GALNT2 antibody; Lane 1: Hela whole cell lysates (30ug) ; Lane 2: Rabbit control IgG instead of anti-GALNT2 antibody in Hela whole cell lysate; Lane 3: anti-GALNT2 antibody (2ug) + Hela whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GALNT2 antibody at a dilution of 0.5 ug/ml and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. Western blot analysis of GALNT2 shows a ~65 kDa band that appears as a tight doublet in some samples, consistent with differential N-glycosylation and maturation states of this Golgi glycosyltransferase.