Ki-67 Antibody / MKI67

Product Specifications

Specifications

Immunohistochemistry: 1:50-1:200, Immunofluorescence: 1:50-1:200, Immunocytochemistry/Immunofluorescence: 1:50-1:200

UniProt

P46013

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein with human Ki67 (Position: K2860-I3256) was used as the immunogen for the Ki-67 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31K02

Applications

IHC, IF, ICC/IF

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Ki-67 antibody is available for research use only.

Storage Conditions

Store the Ki-67 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Ki-67 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunohistochemical staining of Ki67/MKI67 using anti-Ki-67 antibody. Ki67/MKI67 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-Ki-67 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.