LMOD3 Antibody / Leiomodin 3

After reconstitution, the LMOD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot analysis of LMOD3 using anti-LMOD3 antibody. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMOD3 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A predominant band at ~75 kDa with a weaker band at ~69-70 kDa is detected in skeletal muscle and heart. The upward shift relative to the ~65 kDa prediction and the paired bands are consistent with phosphorylation-dependent mobility differences and the known slower SDS-PAGE migration of LMOD3 due to its low-complexity regions.