APH1A Antibody / Gamma-secretase subunit APH-1A

Product Specifications

Specifications

Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

Q96BI3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human APH1A was used as the immunogen for the APH1A antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

FACS, IF, IHC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This APH1A antibody is available for research use only.

Storage Conditions

After reconstitution, the APH1A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the APH1A antibody should be determined by the researcher.

Location

Cytoplasm (ER, Golgi)

Image Legend

Immunofluorescent staining of APH1A using anti-APH1A antibody (red) . APH1A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/ml rabbit anti-APH1A antibody overnight at 4oC. DyLight 550 Conjugated Donkey Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.