BDKRB2 Antibody / Bradykinin receptor B2
Product Specifications
Specifications
Western Blot: 0.25-0.5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate
UniProt
P30411
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A synthesized peptide derived from human BDKRB2 was used as the immunogen for the BDKRB2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
AFAG-2
Applications
WB, IHC, IP
Purity
Affinity chromatography
Format
Liquid
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.
Limitations
This BDKRB2 antibody is available for research use only.
Storage Conditions
Store the BDKRB2 antibody at -20°C.
Formulation
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the BDKRB2 antibody should be determined by the researcher.
Image Legend
Western blot analysis of BDKRB2 using anti-BDKRB2 antibody. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: mouse lung tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDKRB2 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for BDKRB2 at approximately 78 kDa. The expected molecular weight of BDKRB2 is ~78 kDa.
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