GLUD1 Antibody / Glutamate dehydrogenase 1

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

P00367

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human GLUD1 recombinant protein (Position: A210-T558) was used as the immunogen for the GLUD1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, IP, IF, IHC, ICC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This GLUD1 antibody is available for research use only.

Storage Conditions

After reconstitution, the GLUD1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the GLUD1 antibody should be determined by the researcher.

Location

Cytoplasm (Mitochondria, ER)

Image Legend

Immunohistochemical staining of GLUD1 using anti-GLUD1 antibody. GLUD1 was detected in a paraffin-embedded section of human adrenal adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-GLUD1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.