Phospho-Nucleophosmin (Ser125) Antibody / NPM1

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

UniProt

P06748

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-Nucleophosmin (S125) was used as the immunogen for the Phospho-Nucleophosmin (Ser125) antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32N71

Applications

WB, IHC

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This Phospho-Nucleophosmin (Ser125) antibody is available for research use only.

Storage Conditions

Store the Phospho-Nucleophosmin (Ser125) antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the Phospho-Nucleophosmin (Ser125) antibody should be determined by the researcher.

Image Legend

Western blot testing of human samples using the Phospho-Nucleophosmin (Ser125) antibody at 1:3000 dilution for 1 hour at room temperature. A predominant band is observed at ~35 kDa, consistent with phosphorylated NPM1 migrating slightly above its predicted 33 kDa. An additional ~26 kDa species is detected, consistent with a Ser125-containing proteolytic fragment of NPM1 that appears in some cell lysates.