ICAM1 Antibody / CD54

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200

UniProt

P05362

Host

Rabbit

Reactivity

Mouse

Immunogen

A synthesized peptide derived from human ICAM1 / CD54 was used as the immunogen for the ICAM1 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

32I16

Applications

WB, IHC

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This ICAM1 antibody is available for research use only.

Storage Conditions

Store the ICAM1 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the ICAM1 antibody should be determined by the researcher.

Location

Cell surface

Image Legend

Immunohistochemical staining of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ICAM1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.