MFAP3L Antibody / Microfibril-associated protein 3-like

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, ELISA: 0.1-0.5 µg/mL

UniProt

O75121

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E.coli-derived human MFAP3L recombinant protein (Position: K29-V409) was used as the immunogen for the MFAP3L antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC, IF, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This MFAP3L antibody is available for research use only.

Storage Conditions

After reconstitution, the MFAP3L antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the MFAP3L antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of MFAP3L using anti-MFAP3L antibody (green) . MFAP3L was detected in an immunocytochemical section of human HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-MFAP3L antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.