MTCH2 Antibody / Mitochondrial carrier homolog 2

Product Specifications

Specifications

ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2-4ug/500ug of lysate, Immunofluorescence: 5 µg/mL, Immunohistochemistry: 2-5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL

UniProt

Q9Y6C9

Host

Rabbit

Reactivity

Human, Rat

Immunogen

E.coli-derived human MTCH2 recombinant protein (Position: M1-I303) was used as the immunogen for the MTCH2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, FACS, IP, IF, IHC, ICC, WB

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This MTCH2 antibody is available for research use only.

Storage Conditions

After reconstitution, the MTCH2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the MTCH2 antibody should be determined by the researcher.

Location

Cytoplasm (Mitochondria)

Image Legend

Immunofluorescent staining of MTCH2 using anti-MTCH2 antibody (green) . MTCH2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-MTCH2 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.