TPH2 Antibody / Tryptophan hydroxylase 2

Product Specifications

Specifications

Western blot: 1:500-1:2000, Immunohistochemistry: 1:50-1:200, Immunofluorescence: 1:50-1:200

UniProt

Q8IWU9

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Recombinant protein with human THP2 (Position: M1-I490) was used as the immunogen for the TPH2 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG

Clone

31T01

Applications

WB, IHC, IF

Purity

Affinity chromatography

Format

Liquid

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA.

Limitations

This TPH2 antibody is available for research use only.

Storage Conditions

Store the TPH2 antibody at -20°C.

Formulation

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5 mg/mL BSA

Applications Notes

Optimal dilution of the TPH2 antibody should be determined by the researcher.

Image Legend

Immunohistochemical staining of TPH2 using anti-TPH2 antibody. TPH2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution) . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPH2 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.