BCAR4 Antibody / Breast cancer anti-estrogen resistance protein 4

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

D3DUG6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human BCAR4. was used as the immunogen for the BCAR4 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This BCAR4 antibody is available for research use only.

Storage Conditions

After reconstitution, the BCAR4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the BCAR4 antibody should be determined by the researcher.

Image Legend

Flow Cytometry analysis of 293T cells using anti-BCAR4 antibody. Overlay histogram showing 293T cells stained with (Blue line) . The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-BCAR4 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.