PAG1 Antibody / Cbp / Phosphoprotein associated with glycosphingolipid-enriched microdomains 1

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunocytochemistry/Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9NWQ8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human PAG1 was used as the immunogen for the PAG1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, ICC/IF, FACS

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This PAG1 antibody is available for research use only.

Storage Conditions

After reconstitution, the PAG1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the PAG1 antibody should be determined by the researcher.

Location

Cytoplasm, plasma membrane

Image Legend

Immunofluorescent staining of PAG1 using anti-PAG1 antibody (green) . PAG1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-PAG1 antibody overnight at 4oC. DyLight 488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. The section was counterstained with DAPI nuclear stain (blue) . Visualize using a fluorescence microscope and filter sets appropriate for the label used.