SIRT6 Antibody / Sirtuin 6
Product Specifications
Specifications
ELISA: 0.1-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Immunocytochemistry: 5 µg/mL, Western Blot: 0.25-0.5 µg/mL
UniProt
Q8N6T7
Host
Rabbit
Reactivity
Human
Immunogen
E.coli-derived human SIRT6 recombinant protein (Position: D14-S355) was used as the immunogen for the SIRT6 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, FACS, IF, ICC, WB
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This SIRT6 antibody is available for research use only.
Storage Conditions
After reconstitution, the SIRT6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the SIRT6 antibody should be determined by the researcher.
Location
Nuclear, ER (minor)
Image Legend
Immunofluorescent staining of SIRT6 using anti-SIRT6 antibody (red) and anti-Beta Tubulin antibody (green) . SIRT6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/ml rabbit anti-SIRT6 antibody and mouse anti-Beta Tubulin antibody overnight at 4oC. Cy3 Conjugated Goat Anti-Rabbit IgG and DyLight 488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37oC. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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