PANX3 Antibody / Pannexin 3

Product Specifications

Specifications

Western Blot: 0.25-0.5 µg/mL, Immunoprecipitation: 2-4ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL

UniProt

Q96QZ0

Host

Rabbit

Reactivity

Human

Immunogen

E.coli-derived human PANX3 recombinant protein (Position: M1-P392) was used as the immunogen for the PANX3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IP, FACS, ELISA

Purity

Immunogen affinity purified

Format

Lyophilized

Buffer

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Limitations

This PANX3 antibody is available for research use only.

Storage Conditions

After reconstitution, the PANX3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml

Applications Notes

Optimal dilution of the PANX3 antibody should be determined by the researcher.

Image Legend

Immunoprecipitating (IP) PANX3 in MCF-7 whole cell lysate. Western blot analysis of PANX3 using anti-PANX3 antibody; Lane 1: MCF-7 whole cell lysates (30ug) ; Lane 2: Rabbit control IgG instead of anti-PANX3 antibody in MCF-7 whole cell lysate; Lane 3: anti-PANX3 antibody (2ug) + MCF-7 whole cell lysate (500ug) . After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PANX3 antibody at a dilution of 0.5 ug/ml and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for PANX3 at approximately 45 kDa. The expected molecular weight of PANX3 is at 45 kDa.